Overview of Kueltz Lab research

The Kueltz lab studies the molecular mechanisms of adaptation to environmental changes, and the evolution of environmental stress tolerance. A primary focus is on osmotic stress (environmental salinity change) in fish, although other studies have examined high osmotolerance in marine intertidal invertebrates and adaptation of daphnia to multiple stressors (pollution and temperature change).
For a more detailed description of research, please see: http://kueltzlab.ucdavis.edu/.

See other coverage of the Kueltz lab: http://kueltzlab.ucdavis.edu/Outreach.cfm?ID=2

Organism-Environment Interactions course lecture

In his lecture for the Organism-Environment Interactions course, Dr. Kueltz gave us an overview of the questions his lab addresses. Some of their current work uses two different euryhaline, or highly osmotolerant, species as models- sticklebacks and tilapias. Interestingly, these species also exhibit cross-tolerance, meaning they are extremely tolerant to environmental stress in general. Specifically, research involving these model species aims to: 1) understand the molecular basis for phenotypic differences that consistently evolve in marine anadramous versus freshwater (landlocked) stickleback populations, and 2) pinpoint changes in the tilapia proteome in response to salinity exposure.

Listen to Dr. Kueltz's lecture:


Approaches

One of the main tools that the lab uses is proteomics, and Dr. Kueltz provided a useful ‘how-to’ guide for proteomics novices in his talk. They use two proteomics approaches to determine how protein composition changes in response to environmental change, and to define differences in protein composition among populations:
1) Separate (fractionate) proteins based on isoelectric points
2) Use Ultra High Performance Liquid Chromatography to separate peptides, followed by mass spectrometry to identify the amino acids within each peptide and determine their sequence.
Listen to the audio files for Dr. Kueltz’s talk (above) for more details on these methods.

Other proteomics and molecular analysis resources

UC Davis Molecular Structure Facility at the Genome Center: http://msf.ucdavis.edu/
UC Davis Proteomics Core Facility at the Genome Center: http://proteomics.ucdavis.edu/
You can take samples to these folks for protein/proteomic analysis. These are fee for service facilities.

UC Davis Campus Mass Spectrometry Facility: http://cmsf.ucdavis.edu/
This is an open-access facility, where the PI pays a certain fee each year and two of her graduate students will have access to the
instruments in that facility. Students receive training on the instruments.

Courses taught by Dr. Kueltz

If you are a graduate student interested in a great overview of stress physiology, at from both a neurological and cellular perspective, take Dr. Kueltz’s “Stress Physiology” course (MCP255/ABG255) offered in the spring. A full description of courses taught by Dr. Kueltz is provided here:
http://animalscience.ucdavis.edu/faculty/kueltz/fcourses.htm